Restriction endonucleases cut strands of DNA at particular recognition sites in order to isolate specific genes. In addition to gene isolatation, they also facilitate cloning as well as protein expression. More specifically, restriction endonucleases recognize specific palindromic sequences and cleave a phosphodiester bond on each strand at that sequence. After digestion with a restriction endonuclease the resulting DNA fragments can be separated by agarose gel electrophoresis and their size can be estimated. A restriction map is generated by using the fragment size data to determine the location of the specific endonuclease recognition sequences on the plasmid. Each restriction enzyme requires specific reaction conditions for optimum activity. An early producer of restriction endonucleases on a commercial scale since 1985, MBR still stands as a leader in both quality and variety.

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